I have long thought my OTS doesn't exhibit that 'bloom' you hear on good ones and I do know my PI is not very well balanced, when I set the trimmer I couldn't get it to closer than 12volts. I now have a Sovtek 12ax7LPS, sold as balanced so I need to do this again.
It seems setting it by DC voltage is not the right way to do it. AC voltage on the PI plates is what Andy Fuchs was suggesting. Others suggest that is the right way to get it in the ballpark then do it by ear. The information about maximising the second harmonic was very useful and it should be easy to set by feeding a signal tone into the PI and checking the output using a spectrum analyser (my hotplate will suffice for this, a dummy load and a line out).
I've just found this one
http://www.sillanumsoft.org/prod01.htmand run it on my laptop and it works fine. I already had a 440Hz sample file on my laptop (for fooling my Canon 7D into not using the AGC whilst videoing) and I got a nice clean spike at 440 a smaller one at 880 and a similar one at 1320. I'm not sure whether thats the right frequency to use but I might also try 1000Hz.