Been trying to think of an attack plan for a couple of days besides telling you you've probably made more than one mistake.
A better option has not arisen.
Nik provides ballpark voltages for amp his models in one handy Excel sheet...
http://ceriatone.com/voltage/VoltageChart.xlsYou'll have to flip through the sheet tabs at the bottom until you get to the EF86.
Nothing thing to do is to meticulously blueprint ever connection and make sure you made a good solder joint (cold solder joints are gremlins that create a lot of hassles) and making sure you didn't short any thing.
Print out the layout AND schematic and check off
each component as you check it's connection.
Pull the tubes and check the voltage from the High Tension (HT) winding at the rectifier socket as well as measuring all the heater/filament connections to make sure they are getting power. Put the rectifier in and make sure you're getting correct B+ voltage to the first filter cap. Insert power tubes and check voltages, insert preamp tubes and check voltages.
You can also research light bulb limiter and build one, to use FIRST and check to see it indicates a short somewhere in the power supply.